【產(chǎn)品中文名稱】 | 人可溶性血管細(xì)胞粘附因子1(sVCAM-1)ELISA Kit |
【產(chǎn)品英文名稱】 | Human soluble vasccular cell adhesion molecule 1,sVCAM-1 Elisa Kit | |
【保存溫度】 | 2-8℃,避光保存 | |
【產(chǎn)品規(guī)格】 | 96T/48T | |
【有效期】 | 6個(gè)月 | |
購買本司試劑盒,全程提供技術(shù)指導(dǎo),有質(zhì)量問題免費(fèi)包退換.提供免費(fèi)代測(cè)服務(wù),歡迎. | |
該試劑盒適合檢測(cè)血清,血漿,細(xì)胞上清,組織等樣本,產(chǎn)品靈敏度高,具體見產(chǎn)品說明書. | | |
如果你需要試劑盒的樣本收集處理資料以及試劑盒說明書,索要! | | |
人可溶性血管細(xì)胞粘附因子1(sVCAM-1)ELISA Kit | | | | |
【注意事項(xiàng)】 | | | | | |
1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。 |
2.濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。 | | |
3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。 | | | |
一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。 | | |
4.請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔*孔的OD值), | |
請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定,計(jì)算時(shí)請(qǐng)zui后乘以總稀釋倍數(shù)(×n×5)。 | | |
人可溶性血管細(xì)胞粘附因子1(sVCAM-1)ELISA Kit |
【實(shí)驗(yàn)中zui可能出現(xiàn)問題】 | | | | | |
○一 所有孔未顯色 ? 分析原因: | | |
(1) 微孔加入抗體后,洗板不*,微孔中存在游離的抗體。 | | | | |
(2)用錯(cuò)試劑。 | | | | |
(3) 在操作中忘記加酶或抗體。 ? | | | | |
解決辦法: | | | | | |
(1) 檢查洗板器械,比如洗板機(jī)管道是否堵塞、洗液是否用完。 | | | | |
(2)在加樣前,要看清瓶簽。 | | | | |
(3) 嚴(yán)格按說明書操作步驟進(jìn)行實(shí)驗(yàn),確保每步都到位。 | | | | |
○二 所有孔顯色偏低 ? 分析原因: | |
(1)微孔加入抗體后,洗板不*,微孔中存在游離的抗體。 | | | | |
(2) 洗板不干凈。 | | | | |
(3) 抗體污染了酶結(jié)合物等其它試劑。 | | | | |
(4)溫度沒達(dá)到要求。 | | | | |
(5) 每孔加入的試劑量低于說明書要求量。 ? | | | | |
2.解決辦法: | | |
(1)手工加蒸餾水洗板時(shí),要避免吸頭接觸微孔,常更換新的蒸餾水。 | |
(2) 洗板時(shí)要確保每孔都注滿蒸餾水(約400ul),且至少洗板五次。 | | |
(3)嚴(yán)格按說明書要求操作,每次取樣要更換吸頭。 | | |
(4) 控制孵育溫度在25℃。 | | | |
(5) 加樣器吸頭安放牢固避免吸樣不足,加樣時(shí)務(wù)必將吸頭內(nèi)液體打盡。 | | |
人可溶性血管細(xì)胞粘附因子1(sVCAM-1)ELISA Kit | | | | |
相關(guān)產(chǎn)品 | | | | |
兔子血栓調(diào)節(jié)蛋白(TM)ELISA Kit Rabbit thrombomodulin,TM ELISA Kit 兔血栓素B2(TXB2)ELISA Kit Rabbit Thromboxane B2,TX-B2 ELISA Kit 兔子促甲狀腺素(TSH)ELISA Kit Rabbit Thyroid Stimulating Hormone,TSH ELISA Kit 兔子甲狀腺素(T4)ELISA Kit Rabbit thyroxine,T4 ELISA Kit 兔組織因子途徑抑制物(TFPI)ELISA Kit Rabbit Tissue factor pathway inhibitor,TFPI ELISA Kit 兔子組織因子(TF)ELISA Kit Rabbit Tissue factor,TF ELISA Kit 兔組織因子(TF)ELISA Kit Rabbit Tissue factor,TF ELISA Kit 兔子基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)ELISA Kit Rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit 兔子組織多肽抗原(TPA)ELISA Kit Rabbit Tissue Polypeptide Antigen,TPA ELISA Kit 兔子組織多肽抗原(TPA)ELISA Kit Rabbit Tissue Polypeptide Antigen,TPA ELISA Kit 兔子組織型纖溶酶原激活劑(t-PA)ELISA Kit Rabbit Tissue-type Plasiminogen Actilyse,t-PA ELISA Kit 兔子轉(zhuǎn)化生長因子β1(TGF-β1)ELISA Kit Rabbit Transforming Growth factor β1,TGF-β1 ELISA Kit 兔轉(zhuǎn)化生長因子β2(TGFβ2)ELISA Kit Rabbit transforming growth factorβ2,TGFβ2 ELISA Kit 兔肌鈣蛋白Ⅰ(Tn-Ⅰ)ELISA Kit Rabbit Troponin Ⅰ,Tn-Ⅰ ELISA Kit 兔子腫瘤壞死因子α(TNF-α) ELISA Kit Rabbit Tumor necrosis factor α,TNFα ELISA Kit 兔子腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)ELISA Kit Rabbit tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA Kit 兔子腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)ELISA Kit Rabbit tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA Kit 兔尿激酶型纖溶酶原激活物(uPA)ELISA Kit Rabbit urokinase plasminogen activator,uPA ELISA kit 兔尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)ELISA Kit Rabbit Urokinase-type Plasminogen Activator 兔子可溶性粘附分子(Sam)ELISA Kit Rabbit soluble adhesion molecules,Sam ELISA Kit 兔子可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA Kit Rabbit soluble endothelial protein C receptor,sEPCR ELISA Kit 兔子腎上腺髓質(zhì)素(ADM)ELISA Kit Rabbit soluble endothelial protein C receptor,sEPCR ELISA Kit 兔子可溶性E選擇素(sE-selectin)ELISA Kit Rabbit soluble E-selectin,sE-selectin ELISA Kit 兔可溶性凋亡相關(guān)因子(sFAS/Apo-1)ELISA Kit Rabbit soluble Factor-related Apoptosis,sFAS/Apo-1 ELISA Kit 兔子可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA Kit Rabbit Soluble Intercellular Adhesion Molecule-1,sICAM-1 ELISA Kit 兔子白介素1可溶性受體I (IL-1sR Ⅰ)ELISA Kit Rabbit soluble interleukin-1 receptor Ⅰ,IL-1sR Ⅰ ELISA kit 兔子S100蛋白(S-100)ELISA Kit Rabbit Soluble protein-100,S-100 ELISA Kit 兔子S100B蛋白(S-100B)ELISA Kit Rabbit Soluble protein-100B,S-100B ELISA Kit 兔子可溶性P選擇素(sP-selectin)ELISA Kit Rabbit soluble P-selectin,sP-selectin ELISA Kit 兔子可溶性血管細(xì)胞粘附分子1(sVCAM-1)ELISA Kit Rabbit soluble Vascuolar cell adhesion molecule 1,sVCAM-1 ELISA Kit 兔p物質(zhì)(SP)ELISA Kit Rabbit Substance P,SP ELISA Kit 兔子端粒酶(TE)ELISA Kit Rabbit omerase,TE ELISA Kit 兔子睪酮(T)ELISA Kit Rabbit Testoterone,T ELISA Kit 兔纖溶抑制因子/凝血酶激活的纖溶抑制物(TAFI)ELISA Kit Rabbit Thrombin activatable fibrinolysis inhibitor,TAFI ELISA Kit 兔子凝血酶受體(TR)ELISA Kit Rabbit thrombin receptor,TR ELISA Kit 兔子腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)ELISA Kit Rabbit tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA Kit 兔子腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)ELISA Kit Rabbit tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA Kit 兔尿激酶型纖溶酶原激活物(uPA)ELISA Kit Rabbit urokinase plasminogen activator,uPA ELISA kit 兔尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)ELISA Kit Rabbit Urokinase-type Plasminogen Activator Receptor,PLAUR/uPAR ELISA kit 兔血管內(nèi)皮細(xì)胞生長因子(VEGF)ELISA Kit Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit 兔血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA kit Rabbit Vascuolar cell adhesion molecule 1,VCAM-1 ELISA kit 兔血管活性腸肽(VIP)ELISA Kit Rabbit Vasoactive Intestinal Peptide,VIP ELISA Kit 兔血管性血友病因子/瑞斯托霉素輔因子(VWF)ELISA Kit Rabbit von Willebrand Factor,VWF ELISA Kit 兔子α2抗纖溶酶(α2-AP)ELISA Kit Rabbit α2-Antiplasmin,α2-AP ELISA Kit 兔子β血小板球蛋白/β血栓環(huán)蛋白(β-TG)ELISA Kit Rabbit β-Thromboglobulin,β-TG ELISA Kit 綿羊補(bǔ)體片段3b受體(C3bR)ELISA Kit Sheep Complement fragment 3b rccptor,C3bR ELISA Kit 綿羊促性腺激素釋放激素(GnRH)ELISA Kit Sheep gonadotropin-releasing hormone,GnRH ELISA | |